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Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART.

TitoloSperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART.
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2008
AutoriBungum, Mona, Spanò M., Humaidan Peter, Eleuteri Patrizia, Rescia Michele, and Giwercman Aleksander
RivistaHum Reprod
Volume23
Issue1
Paginazione4-10
Data di pubblicazione2008 Jan
ISSN02681161
Parole chiaveadult, Centrifugation, Density Gradient, chromatin, Cohort studies, DNA, DNA fragmentation, Female, fertilization in vitro, Humans, Insemination, Artificial, Homologous, male, Predictive Value of Tests, pregnancy, pregnancy rate, Reproductive Techniques, Assisted, Sperm Injections, Intracytoplasmic, Spermatozoa, Staining and Labeling, treatment outcome
Abstract

BACKGROUND: The sperm chromatin structure assay (SCSA) parameter DNA fragmentation index (DFI) has been shown to predict in vivo and in vitro fertility. So far most SCSA studies have been based on SCSA analysis performed on neat semen. The aim of this study is to assess whether SCSA analysis of sperm prepared by density gradient centrifugation (DGC) could add more information in regard to the prediction of treatment outcome.

METHODS: The study included 510 assisted reproductive technique (ART) cycles. SCSA was performed in neat semen and post DGC. SCSA results were expressed in terms of DFI and high DNA stainability (HDS) cell fractions. The outcome parameter was clinical pregnancy (CP).

RESULTS: Scatter-plot diagrams demonstrated that for DGC samples, no DFI cut-off values could be set for in vivo or in vitro fertility. In intrauterine insemination, IVF and ICSI groups the mean difference (95% CI) in DFI post DGC between those who achieved CP and those who did not was 0.2% (-1.7 to 2.0%), 0.4% (-1.9 to 2.8%) and 1.3% (-3.1 to 5.9%), respectively, none of these being statistically significant. The corresponding differences for HDS were 0.1% (-1.3 to 1.5%), 0.1% (-0.7 to 0.9%) and 0.6% (-1.6 to 2.7%), respectively (all P-values >0.6).

CONCLUSIONS: SCSA performed in semen prepared by DGC cannot predict the outcome of ART.

Note

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-40549105428&doi=10.1093%2fhumrep%2fdem353&partnerID=40&md5=ee8e89af938c5b110d0db0231faa18b0
DOI10.1093/humrep/dem353
Alternate JournalHum. Reprod.
Citation Key4915
PubMed ID17986484